HSD17B8 Antibody

• Catalog Number: orb1260665
• Category: Antibodies
• Description: HSD17B8 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human HSD17B8 (NP_055049.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 27kDa
• Target: HSD17B8
• UniProt ID: Q92506
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: D6S2245E, FABG, FABGL, H2-KE6, HKE6, KE6, RING2, S
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HSD17B8 Antibody (orb1260665) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of U-2 OS cells using HSD17B8 Polyclonal Antibody (orb1260665) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.