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HRASLS2 Antibody

SKU: orb1246538

Description

HRASLS2 Antibody

Research Area

Signal Transduction

Images & Validation

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Tested ApplicationsELISA, IHC
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx. 21kDa band in Human Kidney lysates after 0.5 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.4kDa according to NP_060348.1 (provisional). The 21kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows a granular pattern in the cytoplasm of hepatocytes. Recommended concentration, 5-10µg/mL.

Key Properties

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Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: QELPYSLTSDNC
TargetHRASLS2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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HRASLS2 , HRAS-like suppressor 2, FLJ20556

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UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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