HRAS Antibody

• Catalog Number: orb1262955
• Category: Antibodies
• Description: HRAS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This HRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-128 amino acids from the Central region of human HRAS.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:500For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 21 kDa
• Target: HRAS
• UniProt ID: P01112
• NCBI: P01112
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein
• Note: For research use only
• Application notes: For WB starting dilution is: 1:500For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysate from 293T cell line, using HRAS Antibody at 1:1000.

Western blot analysis of anti-HRAS Antibody in Jurkat cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with HRAS antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Confocal immunofluorescent analysis of HRAS Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).