HR23A Antibody

SKU: orb345297

Description

HR23A antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:4,000 - 1:16,000, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to HR23A by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 115-140 of human HR23A protein.
• Purity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human HR23A protein. Sequence homology as assessed by BLAST indicated 100% homology for this protein from human and rabbit. Cross reactivity with HR23A protein from dog, mouse and rat may also occur as sequence homology varies by one or two amino acid residues in this sequence by BLAST analysis. Reactivity with HR23A protein from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.2 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

goat anti-HR23A Antibody, hHR23A antibody, MGC111083 antibody, RAD23 homolog A antibody, RAD23 yeast homolog A antibody, RAD23A antibody, UV excision repair protein RAD23 homolog A

Western blot showing Biorbyt's Affinity Purified anti-HR23A antibody detects endogenous human HR23A. Lane 1: HeLa nuclear extract (p/n orb348671). Lane 2: HeLa (p/n orb348668). Lane 3: A431 (p/n orb348665). Lane 4: Jurkat (p/n orb348674). Lane 5: 293 whole cell lysates (p/n orb348669). Comparison to a molecular weight marker (at left) indicates a band of ~60 kDa corresponding to HR23A. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using HRP conjugated Rb-a-Goat IgG (p/n orb346990) and chemiluminescence reagent with a 30-min exposure time. Other detection systems will yield similar results.

Quick Database Links

UniProt

P54725

RefSeq:4826964