HPV16 E6 Rabbit Polyclonal Antibody

• Catalog Number: orb10837
• Category: Antibodies
• Description: HPV16 E6 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HPV16 E6 (85-158/158aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 11 kDa
• Target: L2
• RRID: AB_10752337
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: E6; Human Papilloma Virus; Human papillomavirus ty
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Yu, Lan et al. Disruption of human papillomavirus 16 E6 gene by clustered regularly interspaced short palindromic repeat/Cas system in human cervical cancer cells Onco Targets Ther, 8, 37-44 (2015)

PubMed: 25565864

Applications

WB

Reactivity

Human

Hu, Zheng et al. TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy J Clin Invest, 125, 425-436 (2015)

PubMed: 25500889

Applications

WB

Wang, Qiqi et al. HPV16 E6 promotes cervical cancer cell migration and invasion by downregulation of NHERF1 Int. J. Cancer, (2018)

PubMed: 30230542

Li, Mengtao et al. Exposure to microbial metabolite butyrate prolongs the survival time and changes the growth pattern of HPV16 E6/E7-immortalized keratinocytes in vivo Am J Pathol, (2021)

PubMed: 34214507

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-HPV16 E6 antibody (orb10837), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Tissue/Cell: human cervical carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-HPV16-E6 Polyclonal Antibody, Unconjugated (orb10837) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.