HOXD8 Rabbit Polyclonal Antibody

SKU: orb157574

Description

HOXD8 Rabbit Polyclonal Antibody

Research Area

Cell Signaling, Epigenetics and Nuclear Signaling, Intercellular Signaling, Molecular Biology, Surface Molecules

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HOXD8 (151-250/290aa)
• Target: HOXD8
• Molecular Weight: 32 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

homeo box 4E; homeo box D8; homeobox D8; homeobox protein 5.4; Homeobox protein Hox-4E; Homeobox protein Hox-5.4; Homeobox protein Hox-D8; Hox-4.5; Hox-4.5, mouse, homolog of; Hox-4E; Hox-5.4; HOX4; Hoxd8; HXD8_HUMAN.

Protein: muscle (mouse) lysate at 40 ug, Primary: rabbit Anti-HOXD8 (orb157574) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 32 kD, Observed band size: 34 kD.

Tissue/Cell: Human colon cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-HOXC8 Polyclonal Antibody, Unconjugated (orb157574) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

HOXD8

UniProt

P13378