HOXB5 Rabbit Polyclonal Antibody

• Catalog Number: orb101713
• Category: Antibodies
• Description: HOXB5 Rabbit Polyclonal Antibody
• Target: HOXB5
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Equine, Porcine, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human HOXB5 (181-269/269aa)
• UniProt ID: P09067
• MW: 31 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Homeo box 2A; Homeo box B5; Homeobox 2A; Homeobox
• Research Area: Epigenetics and Nuclear Signaling
• Note: For research use only

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-HOXB5 antibody (orb101713), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Spinal cord (Mouse) Lysate at 40 ug, Primary: Anti-HOXB5 (orb101713) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.