HOXB5 Rabbit Polyclonal Antibody

SKU: orb101713

Description

HOXB5 Rabbit Polyclonal Antibody

Research Area

Epigenetics and Nuclear Signaling

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Equine, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HOXB5 (181-269/269aa)
• Target: HOXB5
• Molecular Weight: 31 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Homeo box 2A; Homeo box B5; Homeobox 2A; Homeobox B5; Homeobox protein HHO.C10; Homeobox protein Hox B5; Homeobox protein Hox-2A; Homeobox protein Hox-B5; Homeobox protein HoxB5; Homeobox protein Hu-1; Homeobox2A; HomeoboxB5; HOX 2; Hox 2.1; HOX 2A; HOX B5; HOX2; Hox2.1; HOX2A; HOXB 5; Hoxb5; HU 1; HU1; HXB5_HUMAN.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-HOXB5 antibody (orb101713), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Spinal cord (Mouse) Lysate at 40 ug, Primary: Anti-HOXB5 (orb101713) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Quick Database Links

Gene Symbol

HOXB5

UniProt

P09067