Horse E2(Estradiol) ELISA Kit

• Catalog Number: orb1946548
• Category: Assays and Kits
• Description: Horse E2(Estradiol) ELISA Kit
• Reactivity: Equine
• Range: 15.63-1000 pg/mL
• Target: E2
• Sensitivity: 4.72 pg/mL
• Alternative names: 17B-Estradiol; Oestradiol; Beta-Estradiol
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Estradiol(E2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Estradiol(E2). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estradiol(E2) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.