HNF4A antibody

• Catalog Number: orb19044
• Category: Antibodies
• Description: Goat polyclonal antibody to HNF4A
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Porcine
• Dilution range: ELISA: 1:32000, WB: 0.1-0.5 μg/ml, IHC-P: 2 μg/ml
• Conjugation: Unconjugated
• MW: 51.6; 52.8; 46.6
• Target: HNF4A
• Entrez: 3172
• Protein Sequence: RLSKTLVDMDMADY
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti HNF4A antibody, anti TCF antibody, anti HNF4
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 52kDa and 48kDa bands observed in lysates of Human hepatocellular carcinoma cell line HepG2 (calculated MW of 52.8kDa according to NP_000448.3 and 46.6kDa according to NP_849181.1). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19044 (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Small Intestine using HNF4A antibody

Western blot analysis of Mouse Small Intestine lysate using HNF4A antibody