HNF4A antibody

• Catalog Number: orb216128
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HNF4 alpha (Phospho-S313)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S313 of human HNF4 alpha protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1-200, IF/ICC: 1:100-1:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HNF4A
• Entrez: 25735, 15378, 3172
• UniProt ID: P41235, P22449, P49698
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti HNF4 antibody, anti NR2A1 antibody, anti TCF1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HNF4 alpha (Phospho-S313) expression in HepG2 (A), LO2 (B), C6 (C), CY26 (D) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 52 kD)

Immunohistochemical analysis of HNF4 alpha (Phospho-S313) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of HNF4 alpha (Phospho-S313) staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Direct ELISA antibody dose-response curve using Anti-HNF4 alpha (Phospho-S313) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.