HMGB1 Antibody

• Catalog Number: orb1274463
• Category: Antibodies
• Description: HMGB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 29kDa
• Target: HMGB1
• UniProt ID: P09429
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HMGB1 Antibody: HMG1, HMG3, SBP-1, HMG1, High mobi
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HMGB1 antibody (orb1274463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Western blot analysis of extracts from normal (control) and HMGB1 knockout (KO) 293T cells, using HMGB1 antibody (orb1274463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat brain using HMGB1 Antibody (orb1274463) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 Antibody (orb1274463) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using HMGB1 Antibody (orb1274463) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using HMGB1 antibody (orb1274463) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.