HMCES Antibody

• Catalog Number: orb1520629
• Category: Antibodies
• Description: HMCES Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 304 and 354
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 40 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: HMCES
• UniProt ID: Q96FZ2
• NCBI: Q96FZ2.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: 5-hydroxymethylcytosine (hmC) binding, ES cell-spe
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human HMCES by western blot. Samples: Whole cell lysate (50 µg) from MCF-7, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-HMCES antibody (orb1520629) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human HMCES by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-HMCES antibody (orb1520629) used for IP at 2 µg per reaction.