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Catalog Number | orb1520628 |
---|---|
Category | Antibodies |
Description | HMCES Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | Between 304 and 354 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 40 µl/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | HMCES |
UniProt ID | Q96FZ2 |
NCBI | Q96FZ2.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | 5-hydroxymethylcytosine (hmC) binding, ES cell-spe Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human HMCES by western blot. Samples: Whole cell lysate (50 µg) from MCF-7, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-HMCES antibody (orb1520628) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human HMCES by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-HMCES antibody (orb1520628) used for IP at 2 µg per reaction.
WB | |
Animal, Bovine, Canine, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish | |
Canine, Equine, Human, Mouse, Porcine, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
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