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HLX1 rabbit pAb

SKU: orb765413
FeaturedFeatured Product

Description

Rabbit polyclonal antibody to HLX1

Images & Validation

Tested ApplicationsELISA, IF, IHC-P, WB
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
ReactivityHuman, Mouse, Rat

Key Properties

ClonalityPolyclonal
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human HLX1. AA range:321-370
Molecular Weight75
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

Anti-HLX antibody, anti-HLX1 antibody, anti-H2.0-like homeobox protein antibody, anti-Homeobox protein HB24 antibody, anti-Homeobox protein HLX1 antibody

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HLX1 rabbit pAb

Western Blot analysis of various cells using HLX1 Polyclonal Antibody diluted at 1:1000.

HLX1 rabbit pAb

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

HLX1 rabbit pAb

Immunofluorescence analysis of MCF7 cells, using HLX1 Antibody. The picture on the right is blocked with the synthesized peptide.

HLX1 rabbit pAb

Western blot analysis of lysates from COLO205 cells, using HLX1 Antibody. The lane on the right is blocked with the synthesized peptide.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

HLX1 rabbit pAb (orb765413)

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50 μl
$ 250.00
100 μl
$ 380.00
DispatchUsually dispatched within 5-10 working days
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