HLA-G antibody (Biotin)

• Catalog Number: orb44436
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-G.
• Clonality: Monoclonal
• Clone Number: MEM-G/9
• Tested applications: ELISA, FC, ICC, IHC-Fr, IP
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-5 μg/ml; positive control: JEG-3 human choriocarcinoma cell line. Immunocytochemistry: Recommended dilution: 2-5 μg/ml. For fixation details see: Emadi et al., Biotech Histochem. 2022 Feb;97(2):136-142. Immunohistochemistry: Recommended dilution: 5-10 μg/ml.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: HLA-G
• Entrez: 3135
• UniProt ID: P17693
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-G antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml; positive control: JEG-3 human choriocarcinoma cell line.
• Expiration Date: 12 months from date of receipt.

Separation of HLA-G transfected LCL cells (red) from K562 cells (blue) in flow cytometry analysis (surface staining) using anti-human HLA-G (MEM-G/9) biotin antibody (concentration in sample 4 µg/ml) streptavidin APC.