HLA-G antibody (Biotin)

• Catalog Number: orb43625
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-G.
• Clonality: Monoclonal
• Clone Number: 87G
• Tested applications: ELISA, FC, IHC-Fr
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-3 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: HLA-G
• Entrez: 3135
• UniProt ID: P17693
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-G antibody
• Note: For research use only
• Application notes: Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-3 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of HLA-G transfectants (red) compared with K562 cells (orange) and blank (blue), with anti-HLA-G antibody (87G) biotin / streptavidin-PE.