HLA-DRA Rabbit Polyclonal Antibody (PE)

• Catalog Number: orb124658
• Category: Antibodies
• Description: HLA-DRA Rabbit Polyclonal Antibody (PE)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: PE
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Sheep
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human HLA-DRA (1-100/254aa)
• Tested applications: IF
• Dilution range: IF=1:500-2000
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: DR alpha chain precursor; DRB1; DRB4; HLA class II
• Note: For research use only

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-HLA-DR antibody, Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.