HLA-DR antibody

• Catalog Number: orb198053
• Category: Antibodies
• Description: Mouse Monoclonal antibody to HLA-DR
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 423L
• Tested applications: FACS
• Isotype: IgG2a
• Immunogen: HLA-DR=Derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human lymphoblastoid B-cell line RPMI 8866
• Concentration: See vial for concentration
• Form/Appearance: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
• UniProt ID: P04233
• Storage: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
• Buffer/Preservatives: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Alternative names: HLA-DR*
• Note: For research use only
• Application notes: PBMC: Add 10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Peripheral blood lymphocytes using HLA-DR antibody