HLA-DPB1 Rabbit Polyclonal Antibody

• Catalog Number: orb5450
• Category: Antibodies
• Description: HLA-DPB1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HLA-DPB1 (101-210/258aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 29 kDa
• Target: HLA-DPB1
• UniProt ID: P04440
• RRID: AB_10919288
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: HLA-DP beta1; MHC-2; MHC2; MHC 2; DMA; DMB; DP bet
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-MHC Class II/HLA DMB/AF647 Conjugated antibody, dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-AF647. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-MHC Class II/HLA DMB (orb5450) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 29 kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-HLA DMB (orb5450) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 29 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MHC Class II/HLA-DPB1 Polyclonal Antibody, Unconjugated (orb5450) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.