HLA-C Antibody

• Catalog Number: orb251560
• Category: Antibodies
• Description: HLA-C Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HLA-C (82-106aa EYWDRETQKYKRQAQADRVNLRKLR).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry , 0.5-1μg/ml, Human, - Immunofluorescence, 2μg/ml, Human, - Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 41 kDa
• UniProt ID: Q5QT33
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: WB: The detection limit for HLA-C is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-HLA-C antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of HLA-C using anti-HLA-C antibody.Lane 1:human A431 cell; 2:human 293T cell; 3:human Jurkat cell;4:human U20S cell;5:human HeLa cell;6:human HepG2 cell.

IF analysis of HLA-C using anti-HLA-C antibody.HLA-C was detected in paraffin-embedded section of human Lung cancer tissues.

IF analysis of HLA-C using anti-HLA-C antibody.HLA-C was detected in paraffin-embedded section of human oesophagus squama cancer tissues.

IHC analysis of HLA-C using anti-HLA-C antibody.HLA-C was detected in paraffin-embedded section of Human Lung Cancer Tissue.

IHC analysis of HLA-C using anti-HLA-C antibody.HLA-C was detected in immunocytochemical section of HELA Cell.