HIV-1 Envelope protein

• Catalog Number: orb85310
• Category: Proteins
• Description: This antigen is an E.coli-derived recombinant protein that composes all of the reported immunogenic determinants found in gp41 and at the C-terminus of gp120. The gene encoding this fusion protein was synthesized using codons optimized for E.coli expression and doesn't represent a linear HIV-1 envelope sequence. HIV-1 is a non-glycosylated, 233 amino acid polypeptide chain, having a molecular mass of 27.28 kDa. Superior diagnostic reagent for HIV-1 and HIV type-O detection. Detects all HIV-1 and HIV-type O infected individuals responding to envelope proteins. The fusion protein was purified by proprietary chromatographic technique.
• Clonality: Recombinant
• Form/Appearance: liquid (Supplied in 0.5x PBS containing 6 M urea)
• Purity: > 95% (HPLC)
• Storage: store at -20 °C
• Background: HIV belongs to the retrovirus family, distinguished by possession of a viral reverse transcriptase that transcribes viral RNA into DNA which is integrated into the host-cell genome. The envelope glycoprotein gp160 from HIV, containing two non-covalently associated subunits, gp120 and gp41, mediates the membrane fusion activity of the virus. The surface subunit gp120 attaches to the receptor (CD4) and the coreceptor (CCR5 or CXCR4) on the cell surface, and subsequent conformational changes within the Env complex lead to membrane fusion mediated by the transmembrane subunit gp41.
• Note: For research use only
• Application notes: May be used in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
• Expiration Date: 6 months from date of receipt.