Histone H4 (Acetyl Lys8) rabbit pAb

• Catalog Number: orb763967
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H4 (Acetyl Lys8)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys8. AA range:1-50
• MW: 11
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-HIST1H4A antibody, anti-H4/A antibody, anti-H
• Note: For research use only

Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys8) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 Monoclonal Antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of various cells using Acetyl-Histone H4 was diluted at 1:20000.

Western Blot analysis of KB cells using Acetyl-Histone H4 was diluted at 1:20000.

Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys8) Antibody. The picture on the right is blocked with the synthesized peptide.