HIST3H3 Antibody

SKU: orb572444

Description

This Histone H3K4me1 Antibody is an unconjugated polyclonal product. It tagets HIST3H3 using a A synthetic methylated peptide corresponding to residues surrounding K4 of Human histone H3 as the immunogen. This antibody is suitable for ELISA, IF, IHC, WB. Purification: Antigen Affinity Purified.

Research Area

Epigenetics & Chromatin

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, IHC, WB
Dilution range	WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF: 1:50-1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A synthetic methylated peptide corresponding to residues surrounding K4 of Human histone H3
Target	HIST3H3
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	liquid
Buffer/Preservatives	Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Disclaimer	For research use only

Alternative Names

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HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3

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Immunofluorescence analysis of 293T cell using Histone H3K4me1 antibody.

Western blot analysis of HeLa cell line and H3 protein expressed in E.coli using Histone H3K4me1 antibody.

Dot-blot analysis of methylation peptides using Histone H3K4me1 antibody.

Quick Database Links

Gene Symbol

HIST3H3

UniProt

Q16695

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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