Histone H3 (tri methyl K36) Rabbit Polyclonal Antibody

• Catalog Number: orb6154
• Category: Antibodies
• Description: Histone H3 (tri methyl K36) Rabbit Polyclonal Antibody
• Target: H3C1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Drosophila, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of tri methyl K36 GV(Tri Methyl-K)KP
• UniProt ID: P68431
• RRID: AB_10937841
• MW: 15 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Histone H3(Tri Methyl Lys36); H3K36me3; Tri methyl
• Research Area: Epigenetics and Nuclear Signaling
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Histone H3 (tri methyl K36) antibody (orb6154), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (tri methyl K36)) Polyclonal Antibody, Unconjugated (orb6154) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: SiHa (Human) Cell Lysate at 30 ug, Lane 2: MCF-7 (Human) Cell Lysate at 30 ug, Lane 3: U-2OS (Human) Cell Lysate at 30 ug, Lane 4: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-Histone H3 (tri methyl K36) (orb6154) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kD, Observed band size: 17 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Histone H3 (tri methyl K36) Polyclonal Antibody, Unconjugated (orb6154) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Histone H3 (tri methyl K36) Polyclonal Antibody, Unconjugated (orb6154) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.