Histone H3 / H3-3A antibody

• Catalog Number: orb2276430
• Category: Antibodies
• Description: Histone H3 / H3-3A antibody validated for immunohistochemistry on 76 different Normal Tissues
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human H3.3 histone A fragment
• UniProt ID: P84243
• Tested applications: IHC
• Dilution range: 1:100-1:200
• Application notes: Positive Control: Lymph node: A strong nuclear Histone H3 staining should be seen in all cells while strongest staining intensity occurs in cells of germinal centres. Negative Control: Colon, Kidney: Histone H3 staining should be seen in nuclei only. Cytoplasmic staining should not be seen. Cellular Localization: Intracelullar Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Clone Number: Biorbyt-903M
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3.3 Histone A, H3.3A, H3F3A, H3F3, H3 Histone Fam
• Note: For research use only

Clear cell renal cell carcinoma with strong Histone H3 staining of tumor cells.

Colon mucosa with distinct nuclear Histone H3 staining.

Kidney with Histone H3 staining of nuclei.