Histone H3 (acetyl K4) Rabbit Polyclonal Antibody

• Catalog Number: orb783293
• Category: Antibodies
• Description: Histone H3 (acetyl K4) Rabbit Polyclonal Antibody
• Target: H3C1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthesised acetylpeptide derived from human Histone H3 around the acetylation site of K4 RT(Acetyl-K)QT
• UniProt ID: P68431
• MW: 15 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3K4ac; Histone H3(acetyl Lys4); Acetyl-Histone H3
• Research Area: Epigenetics and Nuclear Signaling
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (acetyl K4)) Polyclonal Antibody, Unconjugated (orb783293) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: K562 (Human) Cell Lysate at 30 ug, Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 3: Molt-4 (Human) Cell Lysate at 30 ug, Primary: Anti-Histone H3 (acetyl K4) (orb783293) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 15 kD, Observed band size: 17 kD.