Histone H2B/HIST1H2BD Rabbit Polyclonal Antibody

SKU: orb6151

Description

Histone H2B/HIST1H2BD Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Histone H2B (21-126/126aa)
• Target: H2BC3
• Molecular Weight: 14 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H2B.1; H2B.1 B; H2B.b; H2B.c; H2B.d; H2B.e; H2B.f; H2B.j; H2B.q; H2BFB; H2BFC; H2BFD; H2BFE; H2BFF; H2BFJ; H2BFO; H2BFQ; H2BFS; HIRIP2; HIST1H2BB; HIST1H2BD; HIST1H2BH; HIST1H2BL; HIST1H2BM; HIST1H2BN; HIST2H2BE; Histone H2B; Histone H2B type 1 B; Histone H2B type 1 D; Histone H2B type 1 H; Histone H2B type 1 L; Histone H2B type 1 M; Histone H2B type 1 N; Histone H2B type 2 E; Histone protein; H2B GL105; H2B histone family member O; H2B histone family member S; histone H2B type 1-B; H2B1B_HUMAN.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-HIST1H2BD antibody (orb6151), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H2B/HIST1H2BD) Polyclonal Antibody, Unconjugated (orb6151) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

H2BC3

UniProt

P33778