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Catalog Number | orb386252 |
---|---|
Category | Antibodies |
Description | Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. [UniProt] |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | HH1/1784R |
Tested applications | FACS, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG, kappa |
Immunogen | Recombinant full-length human protein was used as the immunogen for the rabbit monoclonal recombinant Histone H1 antibody. |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant Histone H1 antibody is available for research use only. |
UniProt ID | P07305 |
Storage | Store the recombinant Histone H1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human pancreas using Histone H1 antibody
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Bovine, Canine, Equine, Hamster, Rabbit | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, ELISA, ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, ELISA, IF, IHC, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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