HIST2H2BE antibody

• Catalog Number: orb340802
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HIST2H2BE
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human Histone H2B
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HIST2H2BE
• Entrez: 319190, 8349
• UniProt ID: Q64524, Q16778
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: H2BFQ; Histone H2B type 2-E; Histone H2B-GL105; Hi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H2B expression in SW480 (A), BT474 (B) whole cell lysates. (Predicted band size: 13 kD; Observed band size: 13 kD)

Immunohistochemical analysis of Histone H2B staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.