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Catalog Number | orb1258367 |
---|---|
Category | Antibodies |
Description | HIST2H2BE Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human HIST2H2BE (NP_003519.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 15kDa |
Target | HIST2H2BE |
UniProt ID | Q16778 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | H2B, H2BQ, GL105, H2B.1, H2BFQ, H2BGL105 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using Histone H2B antibody (orb1258367) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human esophagus using Histone H2B antibody (orb1258367) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using Histone H2B antibody (orb1258367) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using Histone H2B antibody (orb1258367) at dilution of 1:100 (40x lens).
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Unconjugated |
ICC, IF, WB | |
Human, Mouse, Rat | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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