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HIST1H4A (Ab-12) Antibody
Description
Images & Validation
−| Tested Applications | ChIP, ELISA, IF, IHC, IP, WB |
|---|---|
| Dilution range | WB:1:500-5000, IHC-P:1:20-200, IF:1:50-200, IP:1:200-2000 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Peptide sequence around site of Lys (12) derived from Human Histone H4 |
| Target | HIST1H4A |
| Purification | Antigen Affinity Purified |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol |
| Disclaimer | For research use only |
Alternative Names
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Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- HIST1H4A (Ab-12) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time

Western blot aanalysis of Mouse kidney tissue, Mouse brain tissueusing HIST1H4A (Ab-12) antibody

Immunoprecipitation analysis of Mouse Brain tissue using HIST1H4A (Ab-12) antibody

Immunofluorescent analysis of Hela cells using HIST1H4A (Ab-12) antibody

Immunohistochemistry of paraffin-embedded human cervical cancer tissue using HIST1H4A (Ab-12) antibody

Immunohistochemistry of paraffin-embedded human colon cancer tissue using HIST1H4A (Ab-12) antibody
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UniProt Details
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Protocol Information
HIST1H4A (Ab-12) Antibody (orb416524)
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