HiScript II 1st Strand cDNA Synthesis Kit

• Catalog Number: R211-01
• Category: Assays and Kits
• Description: This product is 1st strand cDNA synthesis kit based on Vazyme's HiScript II Reverse Transcriptase. It contains all the components necessary for high quality first strand cDNA synthesis, and the product is suitable for subsequent PCR, qPCR and other experiments. The 2 × RT Mix contains Cu2+ an optimized buffer and dNTPs. The HiScript II Enzyme Mix contains the HiScript II Reverse Transcriptase and the RNase inhibitor. Oligo (dT)23VN has stronger anchoring ability for poly A+ mRNA than Oligo (dT)18, which makes reverse transcription more efficient. Oligo (dT)23VN, Random primers or Gene Specific Primers (GSP) can be selected as reverse transcription primers according to requirements. The kit can be used to synthesize full-length cDNA (up to 20 kb) for cloning and other downstream experiments, as well as highly uniform cDNA for qPCR.
• Storage: Store at -30 ~ -15°C and transport at ≤0°C.
• Note: For research use only
• Application notes: Reliable reverse transcription efficiency: It has strong temperature tolerance (50 ~ 55°C), ensuring that the complex secondary structure of RNA can be opened under high temperature conditions to obtain a longer cDNA. Broad template RNA input: Up to 15 kb cDNA can be obtained from 1 pg - 5 μg total RNA. High cDNA synthesis efficiency: Speicific Anchored Oligo(dT)23VN, ensuring success and efficiency of cDNA synthesis. Selectable primers: Anchored Oligo(dT)23VN and Random hexamers are available individually packaged.
• Expiration Date: Please enquire.