His tag Rabbit Polyclonal Antibody

• Catalog Number: orb10813
• Category: Antibodies
• Description: His tag Rabbit Polyclonal Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: All
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: Synthetic peptide: HHHHHH conjugated to KLH
• RRID: AB_10753064
• Tested applications: ELISA, WB
• Dilution range: WB=1:500-2000, ELISA=1:1000-5000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His
• Research Area: 6x his tag, His Tag
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: His-Tagged Fusion Protein Overexpression E.coli Lysate at 4 ug, Primary: Anti-His Tag (orb10813) at 1/2000 ~ 1/5000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.