HIS Tag Antibody

• Catalog Number: orb1927585
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Target: Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Tested applications: WB
• Dilution range: WB: 1:1000, WB: 1:8000, WB: 1:1,000, WB: 1:2000
• Antibody Type: Primary Antibody
• Clone Number: 6AT18
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Note: For research use only

Western blot analysis of lysates from 12tag protein, This demonstrates the His tagged antibody detected the His tagged protein (arrow).

Anti-HIS Tag at 1:8000 dilution + 12-tag lysate. Lysates/proteins at 20 ng per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-HIS Tag at 1:2000 dilution + 12 tag recombinant protein lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-HIS Tag at 1:2000 dilution + Recombinant protein lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution.Observed band size: 42 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.