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Catalog Number | orb168498 |
---|---|
Category | Proteins |
Description | Recombinant of Hirudin protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Hirudin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Source | Pichia Pastoris |
Biological Activity | The specific activity was found to be > 14,000ATU/mg. |
Storage | Stability: Lyophilized Hirudin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Hirudin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Buffer/Preservatives | Each mg of protein was lyophilized from a sterile solution containing 20mM PBS pH-7 and 2% mannitol. |
Note | For research use only |
Application notes | Recombinant & Natural Proteins |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
9 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
34 kDa | |
E.coli |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 96 % by SDS-PAGE and HPLC analyses. | |
6.7 kDa |