HIPK1 Rabbit Polyclonal Antibody

• Catalog Number: orb184130
• Category: Antibodies
• Description: HIPK1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Monkey, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HIPK1 (1-100/1210aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 131 kDa
• Target: HIPK1
• UniProt ID: Q86Z02
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Hipk 1; Hipk1; HIPK1_HUMAN; Homeodomain interactin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HIPK1) Polyclonal Antibody, Unconjugated (orb184130) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Rat Testis tissue lysates, Lane 3: Human HeLa cell lysates, Lane 4: Human 293T cell lysates, Primary: Anti-HIPK1 (orb184130) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 131 kDa, Observed band size: 130 kDa.

Sample: Mcf-7 (Human) Cell Lysate at 40 ug, Primary: Anti-HIPK1 (orb184130) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 131 kD, Observed band size: 131kD.