Hif3a Antibody

SKU: orb345422

Description

HIF3 alpha antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:14,000 - 1:80,000, WB: 1:1,000 - 1:8,000
• Reactivity: Mouse
• Application Notes:
  This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72 kDa in size corresponding to Hif3 alpha protein by western blotting in the appropriate cell lysate or extract. This antibody is expected to cross-react with mouse and rat Hif3 alpha due to sequence homology.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 575-600 of mouse Hif3 a (Hypoxia Inducible Factor).
• Target: Hif3a
• Purity: This affinity-purified antibody is directed against mouse Hif3alpha protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Hif3a protein from mouse (100% homology) and rat (91% homology). Reactivity against human Hif3a is not expected as only 75% homology for the immunizing sequence is noted. No reactivity is expected against other forms of Hif proteins. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Hif3a antibody, Hif3 alpha antibody, Hif3α antibody, Hypoxia Inducible Factor 3-alpha antibody, Hypoxia inducible factor 3 alpha subunit antibody, Inhibitory PAS domain protein antibody, IPAS antibody, HIF-3-alpha, Basic-helix-loop-helix-PAS protein MOP7, HIF3-alpha-1, Neonatal and embryonic PAS protein

Quick Database Links

Gene Symbol

Hif3a

UniProt

Q0VBL6

RefSeq:170295859