HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) Antibody

• Catalog Number: orb2320854
• Category: Antibodies
• Description: HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Ha111a
• Tested applications: ELISA, IHC, WB
• Reactivity: Gallus, Human, Mouse
• Isotype: IgG2a
• Immunogen: Synthetic peptide corresponding to human HIF-1 alpha aa 239-530.
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Dilution range: ELISA (For coating, order antibody without BSA); Western Blot (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 92-110kDa
• Target: HIF1A
• Entrez: 3091
• UniProt ID: Q16665
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ARNT-interacting protein; Basic-helix-loop-helix-P
• Note: For research use only
• Application notes: Positive Control: A431 or U87 cells. Cobalt chloride treated HeLa cells. Breast or bladder carcinomas
• Expiration Date: 12 months from date of receipt.