HGF Rabbit Polyclonal Antibody

SKU: orb10794

Description

HGF Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HGF alpha (201-300/723aa)
• Target: HGF
• Molecular Weight: 54/80 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

HGF alpha; HGFA; F TCF; Hepatocyte growth factor; Fibroblast derived tumor cytotoxic factor; Hepatocyte growth factor precursor; Hepatopoietin A; HPTA; Lung fibroblast derived mitogen; Scatter factor; SF; hepatocyte growth factor alpha-chain; Hepatocyte growth factor alpha chain; HGF_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (HGF) Polyclonal Antibody, Unconjugated (orb10794) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Lane 1: Rat Placenta tissue lysates, Lane 2: Rat Cerebellum tissue lysates, Primary: Anti-HGF (orb10794) at 1/200 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54/80 kDa, Observed band size: 60 kDa.

Quick Database Links

Gene Symbol

HGF

UniProt

P14210

References

Zhao, Yong et al. Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production Theriogenology, 89, 280-288 (2017)

PubMed: 28043364