HFM1 Antibody

• Catalog Number: orb1270715
• Category: Antibodies
• Description: HFM1 Antibody
• Target: HFM1
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This HFM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1293-1321 amino acids from the C-terminal region of human HFM1.
• UniProt ID: A2PYH4
• MW: 163 kDa
• Tested applications: FC, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Probable ATP-dependent DNA helicase HFM1, SEC63 do
• Research Area: Cell Biology
• Note: For research use only
• NCBI: A2PYH4

Western blot analysis in MDA-MB231 cell line lysates (35 ug/lane).

HFM1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.