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Catalog Number | orb1927777 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB22184 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:50~100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 45080 Da |
Target | This HFE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-162 amino acids from the Central region of human HFE2. |
UniProt ID | Q6ZVN8 |
NCBI | NP_998818.1, NP_973733.1, NP_998817.1, NP_660320.3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Hemojuvelin, Hemochromatosis type 2 protein, RGM d Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
HFE2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HFE2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of lysates from human skeletal muscle and heart tissue lysates (from left to right), using HFE2 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.