Hexokinase 1 Rabbit Polyclonal Antibody

SKU: orb5444

Description

Hexokinase 1 Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/test
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Equine, Mouse, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Hexokinase 1 (501-600/917aa)
• Target: HK1
• Molecular Weight: 101 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CNSHA5; HK; HK1-ta; HK1-tb; HK1-tc; HKD; HKI; HMSNR; HXK1; NEDVIBA; NMSR; RP79; hexokinase; Hk-1; Hk1-s; dea; mHk1-s; HEXOKIN; HXK1_HUMAN; HK1; Brain form hexokinase; Hexokinase type I (HK I); Hexokinase-A; 2.7.1.1; HXK1_MOUSE; Hexokinase, tumor isozyme; HXK1_RAT;

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Hexokinase 1) Polyclonal Antibody, Unconjugated (orb5444) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

U-937 cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Hexokinase 1 Antibody (orb5444) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Quick Database Links

Gene Symbol

HK1

UniProt

P19367