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Catalog Number | orb1602466 |
---|---|
Category | Antibodies |
Description | HES1 Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, WB |
Predicted Reactivity | Mouse |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | Recombinant human HES1 protein (1-277/280aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, ELISA=1:5000-10000, ICC/IF=1:50-1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 30 kDa |
Target | HES1 |
UniProt ID | Q14469 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | bHLHb39; C-HAIRY1; c-hairy1A; Class B basic helix- Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Measured by its binding ability in a indirect ELISA. Immobilized Human HES1 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human HES1 Antibody, the EC50 is 178.8 ng/ml.
Sample: Lane 1: Rat Kidney tissue lysates, Lane 2: Rat Liver tissue lysates, Primary: Anti-HES1 (orb1602466) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kDa, Observed band size: 35 kDa.
Sample: Lane 1: Recombinant human HES1 protein, Primary: Anti-HES1 (orb1602466) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kDa, Observed band size: 30 kDa.
FC, ICC, IF, IHC-Fr, IHC-P | |
Bovine, Equine, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, IP, WB | |
Bovine, Equine, Guinea pig, Rabbit, Rat, Zebrafish | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P, WB | |
Canine, Gallus, Mouse, Porcine | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, WB | |
Mouse, Other, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |