HES1 Rabbit Polyclonal Antibody

• Catalog Number: orb1602466
• Category: Antibodies
• Description: HES1 Rabbit Polyclonal Antibody
• Target: HES1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Recombinant human HES1 protein (1-277/280aa)
• UniProt ID: Q14469
• MW: 30 kDa
• Tested applications: ELISA, ICC, WB
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000, ICC/IF=1:50-1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: bHLHb39; C-HAIRY1; c-hairy1A; Class B basic helix-
• Note: For research use only

Measured by its binding ability in a indirect ELISA. Immobilized Human HES1 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human HES1 Antibody, the EC50 is 178.8 ng/ml.

Sample: Lane 1: Rat Kidney tissue lysates, Lane 2: Rat Liver tissue lysates, Primary: Anti-HES1 (orb1602466) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kDa, Observed band size: 35 kDa.

Sample: Lane 1: Recombinant human HES1 protein, Primary: Anti-HES1 (orb1602466) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kDa, Observed band size: 30 kDa.