HES1 Rabbit Polyclonal Antibody

SKU: orb1602466

Description

HES1 Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC
• Dilution Range: ELISA=1:5000-10000, ICC/IF=1:50-200
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human HES1 protein (1-277/280aa)
• Target: HES1
• Molecular Weight: 30 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HAIRY1; HES1; c-Hey1; c-hairy-1; c-hairy1A; HES-1; HHL; HRY; bHLHb39; HES1_CHICK; C-HAIRY1; Hairy and enhancer of split 1; HES1_BOVIN; HES1_HUMAN; Class B basic helix-loop-helix protein 39 (bHLHb39); Hairy homolog; Hairy-like protein (hHL); HL; HES1_MOUSE; HES1_RAT; Hairy-like protein; RHL; hes family bHLH transcription factor 1; hairy homolog (Drosophila); hairy and enhancer of split 1, (Drosophila)

Measured by its binding ability in a indirect ELISA. Immobilized Human HES1 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human HES1 Antibody, the EC50 is 178.8 ng/ml.

Sample: Lane 1: Rat Kidney tissue lysates, Lane 2: Rat Liver tissue lysates, Primary: Anti-HES1 (orb1602466) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kDa, Observed band size: 35 kDa.

Sample: Lane 1: Recombinant human HES1 protein, Primary: Anti-HES1 (orb1602466) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kDa, Observed band size: 30 kDa.

Quick Database Links

Gene Symbol

HES1

UniProt

Q14469