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HES1 Rabbit Polyclonal Antibody

Catalog Number: orb100523

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb100523
CategoryAntibodies
DescriptionHES1 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, ICC, IF, IHC-Fr, IHC-P
Predicted ReactivityBovine, Equine, Porcine, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human HES1 (41-150/280aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW30 kDa
TargetHES1
UniProt IDQ14469
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesbHLHb39; C-HAIRY1; c-hairy1A; Class B basic helix-
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NoteFor research use only
Expiration Date12 months from date of receipt.
HES1 Rabbit Polyclonal Antibody

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-HES1 antibody (orb100523), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HES1 Rabbit Polyclonal Antibody

MCF-7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HES1) polyclonal Antibody, Unconjugated (orb100523) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

HES1 Rabbit Polyclonal Antibody

MCF-7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HES1) polyclonal Antibody, Unconjugated (orb100523) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

HES1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HES1) Polyclonal Antibody, Unconjugated (orb100523) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

HES1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HES1) Polyclonal Antibody, Unconjugated (orb100523) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

HES1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HES1) Polyclonal Antibody, Unconjugated (orb100523) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

HES1 Rabbit Polyclonal Antibody

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-HES1 Polyclonal Antibody, Unconjugated (orb100523) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

HES1 Rabbit Polyclonal Antibody

Tissue/cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-HES1 Polyclonal Antibody, Unconjugated (orb100523) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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