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Catalog Number | orb574166 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to HES1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Predicted Reactivity | Bovine, Equine, Guinea pig, Rabbit, Rat, Zebrafish |
Reactivity | Human, Mouse |
Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human HES1 |
Concentration | 1.0 mg/ml |
Form/Appearance | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Conjugation | Unconjugated |
MW | 30 kDa |
Target | HES1 |
UniProt ID | Q14469 |
Protein Sequence | Synthetic peptide located within the following region: NTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSSR |
NCBI | NP_005515 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Alternative names | HHL, HRY, HES-1, bHLHb39 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. Canonical 30 kDa isoform is identified, and a second isoform of 23 kDa is also present in some samples in N36.
HES1 antibody - N-terminal region (orb574166) validated by WB using Mouse embryo tissue at 1:800.
HES1 antibody - N-terminal region (orb574166) validated by WB using transfected Ls174Tcell lysate at 1 ug/ml.
K562
Rabbit Anti-HES1 Antibody, Paraffin Embedded Tissue: Human cardiac cell, Cellular Data: Epithelial cells of renal tubule, Antibody Concentration: 4.0-8.0 ug/ml, Magnification: 400X.
transfected Ls174T cell lysate at 50 ug/ml.
WB Suggested Anti-HES1 Antibody Titration: 1.25 ug/ml, Positive Control: Raji cell lysate, HES1 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells.
FC, ICC, IF, IHC-Fr, IHC-P | |
Bovine, Equine, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P, WB | |
Canine, Gallus, Mouse, Porcine | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, WB | |
Mouse | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, WB | |
Mouse, Other, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |