Her2 Antibody

• Catalog Number: orb1252076
• Category: Antibodies
• Description: Her2 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2KRI7
• Tested applications: FC, IF, IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for this HER2 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at Room Temp (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER2 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Her2
• UniProt ID: P04626
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: erbB-2 Antibody: NEU, NGL, HER2, TKR1, CD340, HER-
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at Room Temp (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER2 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Expiration Date: 12 months from date of receipt.

IHC testing of breast invasive carcinoma and HER2 antibody (2KRI7).