erbB-2 (Her-2/neu) antibody

• Catalog Number: orb357997
• Category: Antibodies
• Description: Recombinant monoclonal antibody to erbB-2 (Her-2/neu)
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: 4D5-8 (trastuzumab)
• Tested applications: ELISA, FC, IHC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: A431 cells (human epidermoid carcinoma) (over)expressing EGFR.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: erbB-2 (Her-2/neu)
• UniProt ID: P04626
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD340; Metastatic lymph node gene 19 protein (MLN
• Note: For research use only
• Application notes: This antibody binds to erbB-2/Her-2/neu has therapeutic indications particularly in the treatment of breast cancer (c.f. Herceptin). It can also be used to detect HER-2 by flow cytometry or IHC (Glazyrin 2007).
• Expiration Date: 12 months from date of receipt.

IHC-P staining of human breast cancer tissue using erbB-2 antibody (dilution at 5 ug/ml)

Immunohistochemical staining of paraffin embedded human breast cancer tissue using anti-erbB-2 (primary antibody at 5 ug/ml)

Immunohistochemical staining of paraffin embedded human ovary cancer tissue using anti-erbB-2 (primary antibody at 5 ug/ml)

Immunohistochemical staining of paraffin embedded human ovary cancer tissue using anti-erbB-2 (primary antibody at 5 ug/ml)

Immunohistochemical staining of rat lung tissue using anti-erbB-2 (dilution of primary antibody - 5 ug/ml)

Western blot analysis of human lung cancer (lane 1), human breast cancer (lane 2), human ovary cancer (lane 3), U251 cells (lane 4); Hela cells (lane 5) using erbB-2 antibody (2.5 ug/ml)