HELLS antibody

• Catalog Number: orb178580
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HELLS
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein of human HELLS (NP_060533.2).
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200, IP: 1:50-200, ChIP: 1:20-100
• Purity: Affinity purification
• Conjugation: Unconjugated
• MW: 120kDa
• Entrez: 3070
• UniProt ID: Q9NRZ9
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Alternative names: anti LSH antibody, anti PASG antibody, anti SMARCA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Annalisa Tameni 1, Selene Mallia 1, Veronica Manicardi 1, Benedetta Donati 1, Federica Torricelli 1, Emanuele Vitale 1 2, Elisa Salviato 1, Giulia Gambarelli 1, Silvia Muccioli 1, Magda Zanelli 3, Stefano Ascani 4, Giovanni Martino 4 5, Francesca Sanguedolce 6, Elisabetta Sauta 7, Ione Tamagnini 3, Noemi Puccio 1, Antonino Neri 8, Alessia Ciarrocchi 1, Valentina Fragliasso HELLS regulates transcription in T-cell lymphomas by reducing unscheduled R-loops and by facilitating RNAPII progression Nucleic Acids Res, 10, gkae239 (2024)

PubMed: 38597676

Applications

ChIP

Reactivity

Human

Tameni, Annalisa et al. The DNA-helicase HELLS drives ALK- ALCL proliferation by the transcriptional control of a cytokinesis-related program Cell Death Dis, 12, 130 (2021)

PubMed: 33504766

Western blot analysis of extracts of various cell lines using HELLS antibody

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HELLS antibody

Immunohistochemistry of paraffin-embedded mouse testis using HELLS antibody