HELLS Antibody

• Catalog Number: orb1274441
• Category: Antibodies
• Description: HELLS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human HELLS (NP_060533.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 120kDa
• Target: HELLS
• UniProt ID: Q9NRZ9
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: ICF4, LSH, Nbla10143, PASG, SMARCA6, lymphoid-spec
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HELLS antibody (orb1274441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts from normal (control) and HELLS knockout (KO) HeLa cells, using HELLS antibody (orb1274441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded mouse testis using HELLS Antibody (orb1274441) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug HELLS antibody (orb1274441). Western blot was performed from the immunoprecipitate using HELLS antibody (orb1274441) at a dilution of 1:1000.