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Catalog Number | orb2657860 |
---|---|
Category | Tools |
Description | HEK293T/Human SLC7A11 & SLC3A2 Stable Cell Line |
Tested applications | ELISA, FACS |
Tag | C-terminal Flag-tagged & C-terminal 6xHis-tagged |
UniProt ID | P08195, Q9UPY5 |
Storage | Frozen in liquid nitrogen or stored at -80°C |
Buffer/Preservatives | 35% FBS + 55% DMEM + 10% DMSO |
Alternative names | SLC7A11 & SLC3A2 Read more... |
Note | For research use only |
Application notes | Size: 1 vial contains approximately 5x106 cells in 1 ml. Growth Properties: Adherent. Selection Marker: Puromycin & Blasticidin. Culture Medium: DMEM + 10% FBS + 2 μg/mL Puromycin +2 μg/mL Blasticidin |
Expiration Date | 12 months from date of receipt. |
Untransfected HEK-293T cells (green line) and transfected Human-SLC7A11&SLC3A2 HEK-293T stable cells (red line) were stained with anti-SLC7A11 antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
Untransfected HEK-293T cells (green line) and transfected Human-SLC7A11&SLC3A2 HEK-293T stable cells (red line) were stained with anti-Flag antibody (1µg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry. Note: The cells were fixed in 70% ethanol at 4°C overnight.